(These instructions
assume you are familiar with basic Macintosh procedures, if you are not, see a
Macintosh manual or the tutorial which is on most Macintosh computers. Throughout
the following, MB means mouse button.)
MacNMR startup
Go to the MacNMRGN folder (it will usually be open on the desktop,
if it is not open, move the cursor to the MacNMRGN folder icon at the upper
right area of the desktop and click twice).
Open your setup filesgn folder. Open the P31std file, this
will start MacNMR, the NMR acquisition and processing program, and load standard
parameters. When one uses the P31std file, 0 ppm (relative to 85% H3PO4) will
be in the middle of the spectrum and the sweep width is approximately 80 ppm.
If you get a file locked message, move the cursor to OK and click the mouse
button (MB).
Changing the Sample
see Acquisition and Processing of 1D Spectra
Locking and Shimming
see Acquisition and Processing of 1D Spectra
Adjusting the Receiver Gain
see Acquisition and Processing of 1D Spectra
Collecting and Processing the Spectrum
Open the Dashboard by moving the cursor to the Dashboard Button
(top left icon in the MacNMR window). Change the Scans 1D value to 100000. Move
the cursor the Display item at the top of the screen, hold down the MB. If there
is no check mark next to Real Time Display release the MB. If there is a check
mark next to Real Time Display, drag to Real Time Display and then release the
MB. Real Time Display should now be off (there should be no check next to it).
Hold down the apple key and enter z to begin your acquisition. Moniter the progress
of the acquisition by uploading the current FID and then processing it. This
is done in the following way. Move the cursor to the Commands menu item at the
top of the screen, hold down the MB and drag to Upload, release the MB. Again
move the cursor to the Commands menu item, hold the mouse button down and drag
to 1DFT, then release the mouse button. The FID will be converted to a frequency
domain spectrum. See Chapter 4 of the MacNMR manual for information on viewing
and altering the processing commands. When the signal to noise ratio of the
spectrum is satisfactory, abort the acquisition.
Phase Adjustment
see Acquisition and Processing of 1D Spectra
Chemical Shift Referencing and Axis Appearance
see Acquisition and Processing of 1D Spectra
Peak Picking
see Acquisition and Processing of 1D Spectra
Plotting
see Acquisition and Processing of 1D Spectra
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