(These instructions assume you are familiar with basic Macintosh procedures, if you are not, see a Macintosh manual or the tutorial which is on most Macintosh computers. Throughout the following, MB means mouse button.)
Basic Acquisition
Collect a 1D C-13 spectrum (see Acquisition and Processing of 1D C-13 Spectra). Record the RCVR gain and the Scan Count values; they can be found among the parameters listed in the Dashboard. Close the currently active file and open the deptsetup file located in the setup filesgn folder. Open the Dashboard window and set the RCVR gain to the value that you used when collecting the 1D C-13 spectrum, also set Scans 1D to be the same as the Scan Count of the C-13 spectrum. Hold down the Apple key and enter z to begin spectrum acquisition. A dialog box will appear that is used to enter the name of the file which will contain the data and the folder in which the file will be saved. The spectrum collection time will be approximately = Scans1D x 3 x 3.1 sec.
Spectrum Processing and Plotting
Move the cursor to the Commands menu item at the top of the screen. Hold down the option key, hold down the MB and drag to nDFT, release the MB. The Multi-dimensional Filter and Transform Settings dialog box should now be on the screen. Move the cursor to the Load button at the bottom of the dialog box. Click the MB and then go to the setup filesgn folder and open the deptprocess file. Move the cursor to the Apply settings button and click the MB to process the data. Correct the phase of any of the spectra in the set (the first or second spectrum will have all positive peaks, the third spectrum may have positive and/or negative peaks). Go to the nDFT dialog box and make sure the box to the left of Revert to saved near the bottom of the dialog box has a X in it, if it does not, put the cursor in the box and click the MB. Repeat the nDFT command so the phase correction will be applied to all of the spectra. See Chapters 7 and 9 of the Tecmag Manual and Acquisition and Processing of 1D H-1 Spectra for plotting information.