(These instructions assume you are familiar with basic Macintosh procedures, if you are not, see a Macintosh manual or the tutorial which is on most Macintosh computers. Throughout the following, MB means mouse button.)
The kinetics experiments are very similar to the basic single pulse and acquisition experiments. The major difference is that one can collect multiple spectra and one can adjust the time between collection of spectra.
Procedure
Open the standard H1 or C13 setup file (your lab folder should have H1 and C13 setup files, if it does not, find them in the setup filesgn folder), adjust and note the RCVR gain (for directions see H1 or C13 acquisition and processing link).
Close the standard setup file and open the H1 or C13kineticssetup file (located in the setup filesgn folder). Open the Dashboard and set the RCVR gain to the value found above. Move the cursor to the pulse sequence button and click the MB to open the pulse sequence. Move the cursor to the Name: box for EVENT#2 and click the MB. When the EVENT#2 dialog box appears, set the Loop1 value to the desired number of scans for each spectrum (note: all spectra will have the same number of scans and the Scans 1D value which appears in the Dashboard should be 1). The EVENT#7 Delay is the time between scans. The time between spectra is determined by the Loop2 value in EVENT#8 and the Delay value in EVENT#9. The Loop2 value adjustment is analogous to the Loop 1 adjustment described above. The maximum loop value is 65536. Do the following to adjust the EVENT#9 Delay values. Move the cursor to the T2 icon in the pulse sequence, hold down the option key and click the MB. Edit the table that appears so that there is a value for each spectrum. The values can be the same or different. The time between spectra will be the EVENT#9 delay value times the EVENT#8 Loop2 value. When you have finished editing the value table, move the cursor to Write text file and click the MB, move the cursor to Save and click the MB, move the cursor to replace and click the MB, finally move the cursor to OK and click the MB.
Go to the Multidimensional acquisition settings button (fourth button from the left at the top of the pulse sequence) and click the MB. Set Scans 2D to be equal to the number of spectra to be collected. Move the cursor to OK and click the MB. Close the pulse sequence window. Execute the Zero and Go command to start the kinetics experiment. A dialog box will appear that allows you to enter the name and location of the file which will contain the data.
When data collection is complete, open the file containing the data. Hold down the option key and execute the nDFT command. Move the cursor to Load and click the MB. Move the cursor to Template and click the MB. Select the H1 or C13kineticsprocess file in the setup filesgn folder. Move the cursor to Apply Settings and click the MB. If the necessary adjust the spectrum phase. You can then apply the phase settings to all the spectra by repeating the processing as described above, but before applying the settings make sure the Revert to saved item is selected (there should be an x in the box to the left of Revert to saved).